Synthesis and properties of glutathione reductase in stressed peas
Edwards, E.A. | Enard, C. | Creissen, G.P. | Mullineaux, P.M.
We have subjected peas (Pisum sativum L.) to four different oxidative stresses: cold conditions (4 We have subjected peas (Pisum sativum L.) to four different oxidative stresses: cold conditions (4 degrees C) in conjunction with light, treatment with paraquat, fumigation with ozone, and degrees C) in conjunction with light, treatment with paraquat, fumigation with ozone, and illumination of etiolated seedlings (greening). In crude extracts of leaves from stressed plants, illumination of etiolated seedlings (greening). In crude extracts of leaves from stressed plants, an increase (up to twofold) in activity of glutathione reductase (GR) was observed which was an increase (up to twofold) in activity of glutathione reductase (GR) was observed which was consistent with previous reports from several laboratories. In all cases, except for ozone consistent with previous reports from several laboratories. In all cases, except for ozone fumigation, the increase in activity was not due to an elevation in the steady-state levels of GR fumigation, the increase in activity was not due to an elevation in the steady-state levels of GR protein. None of the applied stresses had any effect on steady-state levels of GR mRNA. In protein. None of the applied stresses had any effect on steady-state levels of GR mRNA. In contrast to the small increase in GR activity, the Km of GR for glutathione disulphide showed a contrast to the small increase in GR activity, the Km of GR for glutathione disulphide showed
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