Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene
2004
Kim, C.K. | Chung, J.D. | Park, S.H. | Burrell, A.M. | Kamo, K.K. | Byrne, D.H.
Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.
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