Effect of the addition of different concentrations of activated coal on the <em>in vitro </em>multiplication of yam
García, Misterbino Borges | Tabarez, Yoandris Sosa
Phenolic oxidation can cause serious problems in the establishment, multiplication and survival of explants on most of plant species. The present investigation was developed toevaluate the effect of adding different concentrations of activated charcoal in the culture medium for the in vitro multiplication of uninodal segments of yam (Dioscorea alata L) ‘caraqueño’ clone. Culture medium MS 75% with MS vitamins and sucrose 30 g.l-1 was used. Different concentrations of activated charcoal (0.5, 1.0, 1.5, 2.0 g.l-1) were also added to the culture medium. Number of novo nodes, leaves and length of shoots were determined after 4, 5 and 6 weeks of culture. Results demonstrated that the addition of activated coal had a positive effect to obtain higher number of nodes. Activated charcoal did not affect the number of leaves and length of shoot. Key words: Dioscorea alata L., nodal cuttings, plant tissue culture
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