Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil
Hermenean, Anca | Stan, Miruna | Ardelean, Aurel | Pilat, Luminița | Mihali, Ciprian Valentin | Popescu, Cristina | Nagy, Lajos | Deák, György | Zsuga, Miklós | Kéki, Sándor | Bácskay, Ildikó | Fenyvesi, Ferenc | Costache, Marieta | Dinischiotu, Anca | Vecsernyés, Miklós
This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl₄-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl₄ (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl₄ administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl₄-induced injury in hepatocytes, probably due to its inherent antioxidant properties.
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