Vaccinomics strategy to design an epitope peptide vaccine against Helicobacter pylori
2022
Tamjid, Navid | Eskandari, Sedigheh | Karimi, Zeinab | Nezafat, Navid | Negahdaripour, Manica
Helicobacter pylori is a Gram-negative bacterium estimated to infect about half of the world's population. The world health organization (WHO) has classified this bacterium in the first line of carcinogen agents; however, the only current strategy to deal with this bacterium is using antibiotics. The increase of antibiotic resistance is alarming. Vaccines could be an advantageous approach to overcome this pathogen. In this study, a multi-epitope oral vaccine was designed against H. pylori using computational approaches. GGT, BabA, HP_1453 proteins were used as antigens for finding epitopes. the two CD8+ cytolytic T lymphocyte (CTL) and seven CD4+ helper T lymphocyte (HTL) and B cell immunodominant epitopes were selected using several servers. Then, they were connected to the adjuvant (U-Omp19), COMP, and M-cell motif by GSGSG, KK and EAAAK linkers. Evaluation of the vaccine construct showed it a non-allergen, immunogen, non-toxic, and stable protein. Docking and MD results indicated its proper interaction with TLR4. Based on the obtained results, the designed vaccine, has acceptable mucosal immunogenicity besides stability in the acidic environment. It can be a proper candidate against H. pylori. However, experimental studies should be performed to confirm its stability, efficacy, and safety.
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