Growing Spathiphyllum in vitro: evaluation of some combinations of carbohydrate sources and minerals in the media regarding peroxidase enzyme activity and chlorophyll content
2012
Mosonyi, I.D. | Tilly-Mandy, A. | Stefanovits-Banyai, E.
Two different and previously established in vitro cultures, in vitro axillary shoot and green globular bud (GGb) cultures, of Spathiphyllum ‘Petite’ were evaluated on different media. The GGb form consists of green, globular bud-like tissue that appears to be growing in colonies. The cultures were maintained for 60 days on media containing 0.5 mg/L benzyl-adenine in combination with either 1- or 2-fold MS macroelements and 20 or 40 g/L sucrose. Morphological characteristics, chlorophyll content, dry weight percentages and peroxidase enzyme (POD) activity were measured. Shoot numbers and length was significantly lower in the medium with higher macroelement concentration and sucrose levels, but the dry weight percentage was almost two-fold. The number of leaves was similar but the leaf forms were different: higher sucrose levels resulted more oblong leaf forms. The chlorophyll content was significantly lower in shoots growing in the medium with 40 g/L sucrose and 1-fold MS macroelements than in the others, and the estimated shoot fresh weight was also lower. The POD enzyme activity showed inverse correlation with the chlorophyll content. We conclude that the POD activity rises as the osmotic concentration of the media increases. The multiplication rate of GGbs was lower (4× on average) than normal shoots (9.5× on average).
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