Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification
2017
Choi, Nakyung | Cho, Hyo Jung | Kim, Heejin | Kim, Yangha | Kim, Hak-Ryul | Kim, In-hwan
The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30°C, and the solvent amount of 4mL (based on a substrate weight of 2g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58mol%.
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