Effect of ascorbic acid supplementation on mRNA expression of HSP70 gene in broilers exposed to heat stress
2015
Mishra, Aditya | Patel, Pragati | Singh, H.S. | Goyal, Girraj | Baghel, R.P.S. | Pankaj, P.K.
In the present study, the mRNA expression level of HSP70 in the liver tissue of commercial broilers was investigated in relation to ascorbic acid supplementation in commercial broilers exposed to heat stress. A total of 96 day old Cobb-400 broilers were randomly divided and maintained in comfort (26±1°C) and heat stressed (40±5°C) conditions for 45 days during mid April to May months. Broilers were divided into 4 groups and each group consisting of 6 birds in each replicates. G1 group was kept as control. G2, G3 and G4 group was supplemented with 100, 200 and 300 mg ascorbic acid (AA) respectively. Relative expression analysis of HSP70 in liver tissue was done on day 42ⁿᵈ of the experiment using RT-PCR technique. The mRNA expression in broiler birds was significantly (P<0.01) down-regulated in all the treatment groups as compared to control group in comfort condition, however mRNA expression in broiler birds was significantly (P<0.01) down-regulated in G3 group as compared to other groups in heat stressed condition. In comfort condition, maximum down-regulation (0.41 fold) was found in G3 group followed by G4 group (0.50 fold) as compared to control group. Similarly, in heat stressed condition, maximum down-regulation (0.65 fold) was found in G3 group, followed by G4 group (0.89 fold) in comparison to control group. The present investigation reveal that supplementation of ascorbic acid on the expression patterns of HSP70 gene provide an indication that AA may be useful in combating rigors of heat stress in chickens.
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