Evaluation of Self-Incompatibility Locus Diversity of Domestic Plum (Prunus Domestica L.) using DNA-Based S-Genotyping
2013
Kota-Dombrovska, Irita | Lācis, Gunārs
There is limited information on genetics of self-incompatibility in domestic plum (P. domestica L.). In comparison with other Prunus species, there are no S-allele specific markers for the identification of compatibility groups, and thus, genetic diversity of self-compatibility genes is not clear. Six S-locus-specific markers previously used for other Prunus species were used to study genetics of self-compatibility of plums, and 33 domestic plum cultivars were genotyped. The applied primer pairs showed good transferability among Prunus species and showed high diversity in the tested plant material (14-37 alleles per marker, average observed heterozygocity - 0.953). Applification of the tested primer pairs allowed discrimination of all plum cultivars by unique S-genotypes. Based on the obtained results, primer pairs EM-PC2consFD/ EM-PC3cons RD, PasPcons-F1/ PaC1cons-R1 and F-Box50A/ F-Box intronR are suggested as supplementary markers for characterisation and identification of plum germplasm with potential functional relevance. Although the markers used did not strictly distinguish plum cultivars according to compatibility groups (selfcompatible, partly self-compatible and self-incompatible), group-unique amplification fragments were identified, which can serve as a baseline in further development of specific markers.
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