Potential use of antioxidative mungbean protein hydrolysate as an anticancer asiatic acid carrier
2011
Wongekalak, La-ongdao | Sakulsom, Premwadee | Jirasripongpun, Kalyanee | Hongsprabhas, Parichat
This study investigated the potential use of mungbean (Vigna radiata (L.) Wilczek) protein hydrolysate (MPH) prepared from tryptic hydrolysis as an antioxidative hydrolysate and as a carrier for anticancer asiatic acid (AA). The antioxidant capacity of MPH was 0.67 and 0.46μmol Trolox equivalent (TE)/mg protein, as measured by oxygen radical absorbance capacity-fluorescein (ORACFL) and Trolox equivalent antioxidant capacity (TEAC) assays, respectively. Freeze-drying in lactose excipient reduced the antioxidant capacity of MPH to 0.48μmol TE/mg protein in ORACFL assay (P<0.05) but did not alter antioxidant capacity determined by TEAC assay (P≥0.05). The genotoxicity of H₂O₂ (50μM, 30min) on hepatoblastoma HepG2 could be alleviated after HepG2 cells had taken up MPH after H₂O₂ exposure (P<0.05). Moreover, the inhibition concentration (IC₅₀) of AA in HepG2 was lowered from 58.5μgmL⁻¹ of AA alone to 38.5μgmL⁻¹ when AA was freeze-dried with MPH in lactose excipient (P<0.05). This study suggested that the efficacy of anticancer pentacyclic triterpene AA against hepatoblastoma HepG2 could be increased by co-drying with antioxidative mungbean protein hydrolysate in lactose excipient.
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