Development and Characterization of 47 Novel Microsatellite Markers for Vellozia squamata (Velloziaceae)
2015
Duarte-Barbosa, Marcia | Bajay, Miklos M. | Zucchi, Maria I. | Pivello, Vânia R.
Premise of the study: We developed and validated microsatellite primers for Vellozia squamata (Velloziaceae), an endemic species of the cerrado (Brazilian savannas), to investigate the influence of different fire regimes on its genetic diversity and population structure. Methods and Results: Using a selective hybridization method, we tested 51 SSR loci using a natural population of V. squamata and obtained 47 amplifiable loci. Among these, 26 loci were polymorphic and the average values of genetic diversity were: average number of alleles per locus () = 6.54, average number of alleles per polymorphic locus (ₚ) = 7.13, average observed heterozygosity (ₒ) = 0.22, average expected heterozygosity (ₑ) = 0.49, and average fixation index () = 0.55. Conclusions: These 26 loci allowed us to assess the effects of distinct fire regimes on the genetic structure of V. squamata populations with the aim of establishing strategies for the conservation of this endemic species. The markers can also be useful for future pharmaceutical studies, as the species has great potential for medicinal and cosmetic applications.
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