Vitrification of crabapple, pear, and geum on gellan gum-solidified culture medium
Turner, S.R. | Singha, S.
Shoots of 'Almey' crabapple [Malus baccata (L.) Borkh. X M. pumila var. niedzwetzkyana (Dieck) Schneid.], 'Seckel' pear (Pyrus communis L.), and 'Mrs. Bradshaw' geum (Geum quellyon Sweet.) were cultured on Murashige and Skoog (MS) medium supplemented with 8.8 micromolar BA and containing 0.1% to 0.4% Gelrite. Comparative shoot proliferation and vitrification were determined on Phytagar-solidified medium. Shoot proliferation, culture fresh weight, and vitrification declined in crabapple, and geum with increasing Gelrite concentration. Pear proliferation and fresh weight increased with increasing Gelrite levels, but all shoots were vitrified. There were differences in the vitrification response between pear and the other two genera. The percent dry weight of vitrified cultures on Gelrite-containing media was generally higher than that of nonvitrified cultures on medium containing Phytagar. Vitrification precludes using low Gelrite concentrations for propagating these plants.
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