Regulation of human and mouse procathepsinâE gene expression
2001
Cook, Matthew | Caswell, Richard C. | Richards, Roy J. | Kay, John | Tatnell, Peter J.
CathepsinâE is an intracellular aspartic proteinase that is considered to have a number of physiological roles including antigen processing. Quantitation of procathepsinâE mRNA by LightCycler™ technology indicated that the gene was transcribed in lung but not in kidney of both human and mouse origin. In contrast, the transcript was present in mouse spleen and alveolar macrophages but not in the counterpart tissue/cells from humans. Regulation of human and mouse procathepsinâE gene expression was shown not to be influenced by the extent of CpG methylation but depended on the recognition of potential binding motifs in each promoter region by transcription factors such as GATA1, PU1 and YY1, as revealed by functional analysis using a series of promoter/luciferase reporter gene fusion constructs. Thus the extent to which the procathepsinâE gene is expressed in a particular cell type may depend on the balance between the effects produced by positiveâacting, cellâspecific transcription factors such as GATA1 and PU1 and the negative influence of the ubiquitous YY1 factor. In this way, the relative abundance and influence of general and cellâspecific transcription factors can govern the production of cathepsinâE and thereby account for the sporadic cell and tissue distribution of this enzyme in different species.
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