Implementation of graphitic carbon nitride nanomaterials and laser irradiation for increasing bioethanol production from potato processing wastes
2022
Saeed, Samar | Samer, Mohamed | Mohamed, Mahmoud S. M. | Abdelsalam, Essam | Mohamed, Yasser M. A. | Abdel‑Hafez, Shams H. | Attia, Yasser A.
Agricultural and agro-industrial wastes (e.g., potato peel waste) are causing severe environmental problems. The processes of pretreatment, saccharification, and fermentation are the major obstacles in bioethanol production from wastes and must be overcome by efficient novel techniques. The effect of exposing the fungi (yeast) Saccharomyces cerevisiae to laser source with the addition of graphitic carbon nitride nanosheets (g-C₃N₄) with different concentrations on bioethanol production was investigated through the implementation of a batch anaerobic system and using potato peel waste (PPW). Dichromate test was implemented as quantitative analysis for quantification of the bioethanol yield. The benefits of this test were the appearance of green color indicating the identification of ethanol (C₂H₅OH) by bare eye and the ease to calculate the bioethanol yield through UV–visible spectrophotometry. The control sample (0.0 ppm of g-C₃N₄) showed only a 4% yield of bioethanol; however, by adding 150 ppm to PPW medium, 22.61% of ethanol was produced. Besides, laser irradiations (blue and red) as influencing parameters were studied with and without the addition of g-C₃N₄ nanomaterials aiming to increase the bioethanol. It was determined that the laser irradiation can trigger the bioethanol production (in case of red: 13.13% and in case of blue: 16.14% yields, respectively) compared to the control sample (in absence of g-C₃N₄). However, by adding different concentrations of g-C₃N₄ nanomaterials from 5 to 150 ppm, the bioethanol yield was increased as follows: in case of red: 56.11% and, in case of blue: 56.77%, respectively. It was found that using fungi and exposing it to the blue laser diode source having a wavelength of 450 nm and a power of 250 mW for a duration of 30 min with the addition of 150 mg L⁻¹ of g-C₃N₄ nanomaterials delivered the highest bioethanol yield from PPW.
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