New insights into the formation and recovery of sublethally injured Escherichia coli O157:H7 induced by lactic acid
2022
Lan, Linshu | Shi, Hui
Escherichia coli O157:H7 can be injured by the action of lactic acid (LA) and injured cells can be recovered under suitable condition. In this study, RNA sequencing analysis revealed the overall genes change of sublethally injured (4 mM LA, 60 min; SI) and initial recovered (minA, 20 min; R) cells. Compared with untreated samples, 53 up-regulated and 98 down-regulated differentially expressed genes (DEGs; Pₐdⱼ < 0.05, change fold ≥2) were found in SI. Meanwhile, Genes related to carbohydrate transport and metabolic were up-regulated and the addition of carbohydrate increased cells resistance to LA. Genes involved in osmotic stress response and cell membrane integrity were down-regulated and E. coli O157:H7 cells were sensitive to osmotic stress during sublethal injury. Genes related to iron stress response and cation transport were changed and cation may affect sublethal injury formation by influencing production of ROS and cellular processes. In R, 1370 up-regulated and 1110 down-regulated DEGs were subdivided into various GO terms and membrane, biological adhesion, cell projection, oxidation-reduction process and catalytic activity, etc., showed significant enrichment (corrected P < 0.05). Particularly, genes related to fimbrial, flagellum and type III secretion system were up-regulated, which may improve infection ability and virulence property during recovery of injured cells. These findings provide novel insights into formation and recovery of sublethally injured E. coli O157:H7 induced by LA.
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Эту запись предоставил National Agricultural Library