Ethylenediamine pretreatment of corn stover facilitates high gravity fermentation with low enzyme loading
2018
Zhu, Jia-Qing | Wu, Xiao-Le | Li, Wenzhao | Qin, Lei | Chen, Si | Xu, Tao | Liu, Hong | Zhou, Xiao | Li, Xia | Zhong, Cheng | Li, Bing-Zhi | Yuan, Ying-Jin
This work investigated the effect of ethylenediamine pretreatment on reducing enzyme loading in high gravity fermentation. At optimal conditions of ethylenediamine pretreatment, 85.5% lignin was removed. Enzyme adsorption analysis using a fluorescent cellulose-binding protein showed 35.2% increase of productive adsorption of enzymes to ethylenediamine pretreated biomass, which was caused by high delignification and dramatically increased surface roughness and porosity. In SScF at 15% glucan loading, up to 82.2 g/L ethanol was achieved with a relatively low enzyme loading of 3.6 FPU/g dry matter. It suggested that the remarkably high digestibility of EDA pretreated corn stover could effectively reduce the enzyme loading in the high gravity fermentation of cellulosic ethanol.
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