Liquid chromatographic method for analysis of the naphthalene dicarboxaldehyde derivative of fumonisins
Bennett, G.A. | Richard, J.L.
A liquid chromatographic (LC) method for determining fumonisins in corn, feed, and laboratory cultures was optimized. We investigated the efficiency of extraction solvents, the performance of cleanup columns, the stability of fluorescent derivatives, and the efficiency of LC columns to separate derivatized fumonisins from interferences so that we could develop a sensitive, reliable assay for fumonisins. Maximum extraction efficiency was accomplished by shaking contaminated samples in acetonitrile-water (50 + 50, v/v) for 1 h; methanolwater mixtures and other ratios of acetonitrilewater were less efficient. Solid-phase extraction cleanups on reversed-phase C18 and strong anion exchange (SAX) columns were compared. The SAX columns were very efficient in isolating fumonisins from crude extracts; however, only the C,8 columns could be used to partially purify fumonisin hydrolysis products. A highly fluorescent and stable derivative, prepared from naphthalene-2,3-dicarboxaldehyde, proved to be suitable for LC analysis. Both parent compounds and hydrolysis products were separated in a simple gradient system of acetonitrile-acetic acid and water-acetic acid in less than 30 min.
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