Trypanosoma vivax: A novel method for purification from experimentally infected sheep blood
2005
Gonzalez, L.E. | Garcia, J.A. | Nunez, C. | Perrone, T.M. | Gonzalez-Baradat, B. | Gonzatti, M.I. | Reyna-Bello, A.
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
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