Leaf vernation test for perennial ryegrass
1998
Grabe, D.F.
A supplementary test procedure is described for improving the accuracy of the fluorescence test for perennial ryegrass. In practice, a fluorescence test is conducted as usual. Fluorescent seedlings are then transplanted and grown to the 3- to 4-leaf stage to be identified on the basis of leaf vernation. Leaf vernation refers to the arrangement of the leaves as they emerge from the young shoot-leaves of annual ryegrass are rolled ant leaves of perennial ryegrass are folded. Subtracting the number of fluorescent perennials from the total number of fluorescent seedlings before calculating test fluorescence provides a more accurate determination of the presence of annual ryegrass than does the fluorescence test alone.
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