Production of parthenolide in organ cultures of feverfew
1996
Stojakowska, A. | Kisiel, W.
In vitro culture of Tanacetum parthenium (L.) Sch.Bip was initiated from aseptically germinated seedlings. Shoot culture was derived from nodal explants of the seedlings on MS medium containing 4.44 micromolar (1.0 mg l-1) of 6-benzylaminopurine (BA) and 0.54 micromolar (0.1 mg l-1) of alpha-naphthaleneacetic acid (NAA). Transformed roots were obtained by infection of the stems of aseptically grown seedlings with Agrobacterium rhizogenes LBA 9402. The parthenolide content in the cultivated plant organs was investigated by RP-HPLC. The production of the compound was strongly influenced by the genotype of the parent plant and ranged from 0.13% to 0.75% dry weight in the shoots of the rooted plantlets grown in vitro. The yield of the compound in multiple shoot cultures of T. parthenium reached 60% of that found in the shoots of rooted plantlets. In contrast to shoots, only trace amounts of parthenolide could be detected in some clones of transformed roots and the roots of plantlets.
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