Biological removal of arsenic pollution by soil fungi
2011
Srivastava, Pankaj Kumar | Vaish, Aradhana | Dwivedi, Sanjay | Chakrabarty, D. (Debasis) | Singh, Nandita | Tripathi, Rudra Deo
Fifteen fungal strains were isolated from arsenic contaminated (range 9.45–15.63mgkg⁻¹) agricultural soils from the state of West Bengal, India. Five fungal strains were belonged to the Aspergillus and Trichoderma group each, however, remaining five were identified as the Neocosmospora, Sordaria, Rhizopus, Penicillium and sterile mycelial strain. All these fungal strains were cultivated on medium supplemented with 100, 500, 1000, 5000 and 10,000mgl⁻¹ of sodium arsenate. After 30-day cultivation under laboratory conditions, radial growth of these strains was determined and compared with control. Toxicity and tolerance of these strains to arsenate were evaluated on the basis of tolerance index. Out of fifteen, only five fungal strains were found resistant and survived with tolerance index pattern as 0.956 (sterile mycelial strain)>0.311 (Rhizopus sp.)>0.306 (Neocosmospora sp.)>0.212 (Penicillium sp.)>0.189 (Aspergillus sp.) at 10,000mgl⁻¹ of arsenate. The arsenic removal efficacy of ten fungal strains, tolerant to 5000mgl⁻¹ arsenate, was also assayed under laboratory conditions for 21days. All these strains were cultivated individually on mycological broth enriched with 10mgl⁻¹ of arsenic. The initial and final pH of cultivating medium, fungal biomass and removal of arsenic by each fungal strain were evaluated. Fungal biomass of ten strains removed arsenic biologically from the medium which were ranged from 10.92 to 65.81% depending on fungal species. The flux of biovolatilized arsenic was determined indirectly by estimating the sum of arsenic content in fungal biomass and medium. The mean percent removal as flux of biovolatilized arsenic ranged from 3.71 to 29.86%. The most effective removal of arsenic was observed in the Trichoderma sp., sterile mycelial strain, Neocosmospora sp. and Rhizopus sp. fungal strains. These fungal strains can be effectively used for the bioremediation of arsenic-contaminated agricultural soils.
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