Seed transmission of Melon necrotic spot virus and efficacy of seed-disinfection treatments
2009
Herrera-Vásquez, J.A. | Córdoba-Sellés, M.C. | Cebrián, M.C. | Alfaro-Fernández, A. | Jordá, C.
Rates of seed transmission of Melon necrotic spot virus (MNSV) were estimated in seedlings grown from commercial melon (Cucumis melo) cv. Galia F₁ seeds. Seedlings at the cotyledon stage and adult plants were assayed for MNSV by DAS-ELISA and RT-PCR. None of the seedling groups tested positive for MNSV by ELISA. The proportion of seedlings infected with MNSV was at least 7 and 8% in seed lots 05 and 06, respectively, as estimated from RT-PCR analysis of grouped seedlings. Fourteen and eight grouped samples (10 seedlings per group), of a total of 200 and 100 seedlings, respectively, grown from infected seeds were MNSV-positive in seed lots 05 and 06, respectively, corresponding to seed-to-seedling transmission rates of 11·3 and 14·8%, respectively. Several seed-disinfection treatments were evaluated for their ability to prevent seed transmission of MNSV. The results suggest that a treatment of 144 h at 70°C can be used to eradicate MNSV in melon seeds without hindering germination.
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