Vitamin D<sub>3</sub> and 25-Hydroxyvitamin D<sub>3</sub> Content of Retail White Fish and Eggs in Australia
Dunlop, Eleanor | Cunningham, Judy | Sherriff, Jill L. | Lucas, Robyn M. | Greenfield, Heather | Arcot, Jayashree | Strobel, Norbert | Black, Lucinda J.
Dietary vitamin D may compensate for inadequate sun exposure; however, there have been few investigations into the vitamin D content of Australian foods. We measured vitamin D<inf>3</inf> and 25-hydroxyvitamin D<inf>3</inf> (25(OH)D<inf>3</inf>) in four species of white fish (barramundi, basa, hoki and king dory), and chicken eggs (cage and free-range), purchased from five Australian cities. Samples included local, imported and wild-caught fish, and eggs of varying size from producers with a range of hen stocking densities. Raw and cooked samples were analysed using high performance liquid chromatography with photodiode array. Limits of reporting were 0.2 and 0.1 μg/100 g for vitamin D<inf>3</inf> and 25(OH)D<inf>3</inf>, respectively. The vitamin D<inf>3</inf> content of cooked white fish ranged from <0.1 to 2.3 μg/100 g, and the 25(OH)D<inf>3</inf> content ranged from 0.3 to 0.7 μg/100 g. The vitamin D<inf>3</inf> content of cooked cage eggs ranged from 0.4 to 0.8 μg/100 g, and the 25(OH)D<inf>3</inf> content ranged from 0.4 to 1.2 μg/100 g. The vitamin D<inf>3</inf> content of cooked free-range eggs ranged from 0.3 to 2.2 μg/100 g, and the 25(OH)D<inf>3</inf> content ranged from 0.5 to 0.8 μg/100 g. If, as has been suggested, 25(OH)D<inf>3</inf> has five times greater bioactivity than vitamin D<inf>3</inf>, one cooked serve (100 g) of white fish, and one cooked serve of cage or free-range eggs (120 g) may provide 50% or 100%, respectively, of the current guidelines for the adequate intake of vitamin D (5 µg) for Australians aged 1–50 years.
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