Cryopreservation of white mulberry (Morus alba L.) by encapsulation-dehydration and vitrification
2012
Padrò, Maria Dolores Arias | Frattarelli, Andrea | Sgueglia, Alessandra | Condello, Emiliano | Damiano, C. (Carmine) | Caboni, Emilia
Shoot apices of in vitro-grown plantlets of white mulberry, Morus alba L. cv Florio, were cryopreserved using either encapsulation-dehydration or vitrification. For encapsulation-dehydration, alginate beads containing apices were dehydrated for 1, 3, 5 or 7Â days in a liquid medium containing various sucrose concentrations (0.5, 0.75, 1.0 or 1.25Â M). Bead desiccation was performed using silica gel for either 0, 4, 6, 8, 9 or 14Â h. For vitrification, apices were directly immersed for either 5, 15, 30 or 60Â min in a vitrification solution (PVS2). Following encapsulation-dehydration, treatment of alginate beads with 0.75Â M sucrose was more effective in promoting re-growth of explants after immersion in liquid nitrogen than in the presence of 0.5Â M sucrose for either 1 or 3Â days. Re-growth of explants was also observed following vitrification and this reached 47% with increasing duration of the PVS2 treatment from 5 to 30Â min. Overall, the highest frequency of explant re-growth was obtained when explants were subjected to encapsulation-dehydration in the presence of 0.75Â M along with a 3Â day sucrose dehydration pre-treatment and followed by desiccation for 9Â h in silica gel.
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