Improved membrane permeability with cetyltrimethylammonium bromide (CTAB) addition for enhanced bidirectional transport of substrate and electron shuttles
2022
Wu, Jingxuan | Li, Yunfei | Chen, Xuepeng | Li, Nan | He, Weihua | Feng, Yujie | Liu, Jia
The effects of membrane permeability on extracellular electron transfer (EET) and performance of microbial fuel cell (MFC) need to be explored. In this work, cetyltrimethylammonium bromide (CTAB) was chosen to enhance the current generation and bidirectional transport of substrate and electron shuttles by tailoring the cell membrane permeability. Specifically, the peak currents of biofilms treated with CTAB especially at 200 μM were obviously higher than the control biofilm with no CTAB, and the riboflavin mediated electron transfer was promoted prominently. Biomass and viability analyses showed that an appropriate concentration of CTAB had almost no adverse effect on the cell viability of biofilm and could increase the biomass of biofilm. Measurements of the extracellular activity of alkaline phosphatase and UV–vis absorption confirmed the increased membrane permeability and the promoted efficiency of substrates transported into cells. This contribution paves the key step for facilitating EET process by adjusting membrane permeability through CTAB or other surfactants addition.
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Эту запись предоставил National Agricultural Library