Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera)
Sabater-Muñoz, Beatriz | Legeai, Fabrice | Rispe, Claude | Bonhomme, Joël | Dearden, Peter | Dossat, Carole | Duclert, Aymeric | Gauthier, Jean-Pierre | Giblot Ducray, Danièle | Hunter, Wayne | Dang, Phat | Kambhampati, Srini | Martinez-Torres, David | Cortés Zavala, María Teresa | Moya, Andrés | Nakabachi, Atsushi | Philippe, Cathy | Prunier-Leterme, Nathalie | Rahbé, Yvan | Simon, Jean-Christophe | Stern, David L. | Wincker, Patrick | Tagu, Denis
Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from the pea aphid Acyrthosiphon pisum was carried out to define a catalog of 12,082 unique transcripts. A strong AT bias was found, indicating a compositional shift between Drosophila melanogaster and A. pisum. An in silico profiling analysis characterized 135 transcripts specific to pea-aphid tissues (relating to bacteriocytes and parthenogenetic embryos). This project is the first to address the genetics of the Hemiptera and of a hemimetabolous insect.
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