Radiation-resistant Macrococcus caseolyticus (A) isolated from radiation-processed semidried prawns
2014
Karani, Manisha | Shashidhar, Ravindranath | Kakatkar, Aarti | Gautam, Raj Kamal | Sukhi, Shibani | Pansare-Godambe, Lipika | Bandekar, Jayant
A radiation-resistant bacterial isolate from gamma-radiation-processed (5 kGy) semidried prawns was identified as a new strain of Macrococcus caseolyticus and was designated as M. caseolyticus (A) on the basis of morphological and biochemical characterization and 16S rRNA sequencing. DNA–DNA hybridization studies with M. caseolyticus DSM 20597ᵀ further confirmed the isolate as M. caseolyticus. Major fatty acids present in M. caseolyticus (A) were C14:0, C16:1ω11c, and C18:1ω9c, whereas C15:0anteiso, C16:0iso, and C18:0iso were absent. The closest match for the isolate, as per fatty acid methyl ester analysis, was M. caseolyticus DSM 20597ᵀ. However, the similarity index was significantly low (0.112), which indicates that the isolate could be a new strain of M.caseolyticus. The decimal reduction dose (D₁₀) for M.caseolyticus (A), M.caseolyticus JCSC5402, and Staphylococcusaureus MTCC96 was 1.18, 0.607, and 0.19 kGy, respectively. This is the first report on radiation resistance of M.caseolyticus. Macrococcuscaseolyticus (A) is more resistant to gamma and UV radiation stress than are M.caseolyticus JCSC5402 and S.aureus MTCC96; however, it is sensitive to heat as well as desiccation stress.
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