Potential antioxidative effects of cactus pear fruit (Opuntia ficus-indica) extract on radical scavenging and DNA damage reduction in human peripherial lymphocytes
2006
Siriwardhana, N. | Shahidi, F. | Jeon, YOU-JIN
Cactus pear fruit (Opuntia ficus-indica) extract (CPFE) was investigated for its antioxidative and DNA damage reduction using electron spin resonance spectroscopy and comet assays on human peripheral lymphocytes, respectively. Scavenging of 1,1-diphenyl-2-picrylhydrazyl adducts by CPFE at 0.125, 0.25 and 0.5 mg/mL was 32.5, 56.7 and 71.6%, respectively. CPFE at 0.25 and 0.5 mg/mL scavenged 19.2 and 63.6% of 5,5-dimethyl-1-pyrroline N-oxide-OH adducts. Alkyl radical scavenging effect of CPFE at 0.0625, 0.125, 0.25 and 0.5 mg/mL was 38.0, 42.4, 67.5 and 78.8%, respectively. CPFE reduced the H₂O₂-induced DNA damage in lymphocytes (CPFE at 0.1 mg/mL totally inhibited the damage). The data demonstrate that both antioxidative and DNA damage-reduction activities are increased with increasing CPFE dosage. These results demonstrate the antioxidative and DNA damage-reducing efficacy of CPFE constituents as potential sources of raw material for pharmaceutical and functional food industries.
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