Pulsed-field gel electrophoresis combined with rare-cutting endonucleases for strain differentiation of Candida famata, Kloeckera apiculata and Schizosaccharomyces pombe with chromosome number and size estimation of the two former
1995
Versavaud, A. | Hallet, J.N.
Transverse alternating field electrophoresis combined with digestion of chromosomal DNAs by rare-cutting restriction endonucleases as Not I, Sfi I and Sma I was proves to be efficient for the differentiation of several strains of the yeasts Schizosaccharomyces pombe, Kloeckera apiculata and Candida famata. For these two last genera the chromosome number, estimated from electrophoretic karyotypes, varied within the same species from six to eight. The total size of the genome was next to 10 Mpb for Kl. apiculata and from 14 to 12 Mpb for C. famata.
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