Quantitative determination of tannin and protein in the precipitates by high-performance liquid chromatography
1995
Kawamoto, H. | Nakatsubo, F. | Murakami, K.
High performance liquid chromatography was applied for the determination of both tannin and protein in tannin-protein coprecipitates and the relative affinities of a series of galloylglucoses for bovine serum albumin were determined. Relative affinity is affected mainly by the number of the galloyl groups in the galloylglucose molecule with penta > tetra- > tri- much greater than di- much greater than monogalloylglucose. The position of the galloyl group in the glucose core also affects the relative affinity but the effect is smaller than that of the number with 2,3,6- > 2,3,4-tri-O-galloylglucose and 4,6- > 2,3-di-O-galloylglucose.
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