Determination of the mycotoxin cyclopiazonic acid by enzyme immunoassay
1991
Hahnau, S. | Weiler, E.W.
Antisera highly selective for the mycotoxin cyclopiazonic acid (CPA) were raised in rabbits. A competitive enzyme-linked immunosorbent assay (ELISA) for CPA, with use of alkaline phosphatase as tracer enzyme, was developed. The assay measures from 30 pg to 2 ng of CPA and showed an interassay, between-day variability of 14% and an intraasay variability of 3.7%. An efficient selection technique was devised that allows detection of CPA, even at low levels, in fungal strains growing as agar surface cultures. No precleaning of the samples is required. The technique allows efficient monitoring for fungal isolates producing CPA.
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