Callus induction and organogenesis in wheat/Aegilops longissima chromosome addition lines
2003
Tyankova, N.D. | Zagorska, N.A. | Dimitrov, Bozhidar
The influence of individual Aegilops longissima addition chromosomes on callus induction and organogenesis of in vitro cultivated immature embryos of wheat was studied. Immature 14-day-old embryos of seven Chinese Spring lines with added chromosomes of Aegilops longissima were in vitro cultivated. Although relatively high number of calli were formed in embryo cultures of all lines studied, the different genotypes expressed variation in respect to their response to in vitro cultivation. The number of initiated calli of line D, containing chromosome arms 7S¹L and 4S¹L was relatively low. The lines showed substantial differences in respect to the number of initiated morphogenic calli and regenerants recovered. The highest number of morphogenic calli and regenerants were obtained in cultures of lines containing the chromosomes 2S¹, 6S¹ and to less extend 3S¹.
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