Changes in abscisic acid levels in embryonic axis of Saraca asoca seeds during maturation and artificial dehydration
2017
Prajith, T. M. | Anilkumar, C. | Ajith Kumar, K. G.
Abscisic acid (ABA) plays an important role in many cellular processes, including seed development, dormancy, germination and stress responses. Investigation on changes in endogenous ABA content of Saraca asoca seeds during development and artificial dehydration by indirect competitive ELISA method explains their influence on viability. The low ABA levels towards later stages of seed development validates non-orthodox nature of species. About 37% germination was observed in immature seeds of 60 days after anthesis onwards, which possess comparatively higher ABA content of 0.92 nmol g⁻¹ fr. wt. In the course of seed maturation gradual loss of ABA was noticed with concurrent increase in germination. Artificial seed drying in open laboratory condition for five different periods, viz., 48, 96, 144, 192 and 240 h registered 49.7, 48.4, 46.9, 45.2 and 42.8% moisture contents, respectively, in fresh and mature seeds as control which possess moisture content of 51.6 with 100% germination. Dehydration of seeds results the significant increase of abscisic acid level in embryonic axis but the viability was declined simultaneously. So the present study deals with the influence of abscisic acid in desiccation tolerance during maturation and dehydration of recalcitrant seeds of Saraca asoca.
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