Kinamycin biosynthesis employs a conserved pair of oxidases for B-ring contraction
2015
Wang, Bin | Ren, Jinwei | Li, Liyuan | Guo, Fang. | Pan, Guohui | Ai, Guomin | Aigle, Bertrand | Fan, Keqiang | Yang, Keqian
The biosynthesis of diazobenzofluorene kinamycins requires a hitherto uncharacterized B-ring contraction. Via detailed genetic and enzymatic analyses we unambiguously characterized the conserved pairs of oxidases, AlpJ and AlpK homologs, as nature's machinery for benzofluorenone formation, which paves the way for the investigation of the following diazo assembly.
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