Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers
2004
Shen, Xiaorong | Kong, Lingrang | Ohm, Herbert
The objective of this research was to identify Fusarium head blight (FHB) resistance in wheat (Triticum aestivum)-Lophopyrum genetic lines that might complement FHB resistance in common wheat; and to identify DNA markers that can be used to tag the resistance gene in the alien chromatin (E or el₂ genome) for the development of improved wheat cultivars. FHB resistance was evaluated in 19 Chinese Spring-Lophopyrum elongatum (EE) substitution lines, two Thatcher-L. ponticum (el₁ and el₂) substitution lines, and four Thatcher-L. ponticum translocation lines. Significant resistance was identified in the substitution lines 7E(7A), 7E(7B), and 7E(7D). The homoeologous chromosome, 7el₂,also showed resistance in the Thatcher genetic background. Both the Thatcher-7el₁ substitution and translocation lines were susceptible, like Thatcher, indicating that there is no resistance gene on the 7el₁ chromosome. Simple sequence repeat (SSR) and cleaved amplified polymorphic sequences (CAPS) in homoeologous group 7 chromosomes were used to identify DNA markers located on 7E and 7el₂. As expected, the transferability of wheat SSR markers to Lophopyrum is low. Of the 52 SSR markers that we tested, only five were found to be co-dominant on 7E of L. elongatum versus 7A, 7B, and 7D, one of which is also positive on 7el₂. A CAPS marker, derived from the RFLP probe PSR129, can serve as a dominant marker for 7el₂ chromatin.
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