Neiella holothuriorum sp. nov., isolated from the gut of a sea cucumber Apostichopus japonicus
2022
Bai, Xin-Feng | Lv, Xue-Lian | Liu, Xue | Cui, Ting-Ting | Zhang, Mian-Song | Ding, Ning | Liu, Chang-Heng | Jia, Ai-Rong
A Gram-stain negative, aerobic, rod-shaped bacterium, designated 126ᵀ, was isolated from the intestinal content of a sea cucumber, Apostichopus japonicus, in China. Strain 126ᵀ was found to grow optimally at 25–28 °C and pH 7.5–8.0 in marine 2216 E medium, with tolerance of 1–7% (w/v) NaCl. Strain 126ᵀ is motile by means of one to several polar flagella. The dominant fatty acids of strain 126ᵀ were identified as C₁₆:₁ ω7c/C₁₆:₁ ω6c (29.5%), C₁₈:₁ ω7c/C₁₈:₁ ω6c (19.8%) and C₁₆:₀ (16.7%). The respiratory quinone was found to be Q-8. The polar lipid profile was found to be mainly composed of phosphatidylglycerol and phosphatidylethanolamine. The total length of the draft genome is approximately 4.2 × 10⁶ bp, encoding 3655 genes and 3576 coding sequences. The G + C content of the genomic DNA is 48.0%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 126ᵀ belongs to the genus Neiella and is closely related to Neiella marina J221ᵀ (96.5%). Genomic comparisons of 126ᵀ to N. marina J221ᵀ revealed that they had similar genome size, G + C content and complement of clusters of orthologous groups. However, average nucleotide identity and digital DNA–DNA hybridization values between strains126ᵀ and N. marina J221ᵀ was 75.5% and 19.7%, which could distinguish the strains. On the basis of these phenotypic and genotypic data, strain 126ᵀ is concluded to represent a novel species, for which the name Neiella holothuriorum sp. nov. is proposed. The type strain is 126ᵀ (= GDMCC 1.2530ᵀ = KCTC 82829ᵀ).
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