Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis
2018
Amjad ʻAlī, Muḥammad | Azeem, Farrukh | Nawaz, Muhammad Amjad | Acet, Tuba | Abbas, Amjad | Imran, Qari Muhammad | Shah, Kausar Hussain | Rehman, Hafiz Mamoon | Chung, Gyuhwa | Yang, Seung Hwan | Bohlmann, Holger
Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time PCR, and promoter:GUS lines to analyze expression. Both genes were upregulated in response to abiotic stress. Next, we applied the same stressors to seedlings of T-DNA insertion wrky11 and 17 knock-out mutants (single and double). Under stress, the mutants exhibited slower germination and compromised root growth compared with the wild type. In most cases, double-mutant seedlings were more affected than single mutants. These results suggest that wrky11 and wrky17 are not strictly limited to plant defense responses but are also involved in conferring stress tolerance.
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