Determination of cyclopiazonic acid in food and feeds by liquid chromatography-tandem mass spectrometry
2009
Moldes-Anaya, Angel S. | Asp, Tone N. | Eriksen, Gunnar S. | Skaar, Ida | Rundberget, Thomas
A new, fast and efficient multiple reaction monitoring (MRM) high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of cyclopiazonic acid (CPA) in mixed feed, wheat, peanuts and rice is presented. The analytical methodology involves sample extraction with an alkaline methanol-water mixture, defatting with hexane and quantification using HPLC-MS/MS without further treatment of sample extracts. Reversed-phase liquid chromatography using a C18 stationary phase coupled to negative mode electrospray triple quadrupole tandem mass spectrometry was applied. The limit of detection was 5μg/kg while the limit of quantification was 20μg/kg in the matrices investigated. The detector response was found to be linear over the range 25-250μg/kg in feed and 25-500μg/kg in wheat, peanuts and rice. The mean overall recoveries (n =18) of CPA varied from 79% to 114% in the range of concentrations studied over a period of 4 months. Mean recoveries (n =3 or 6) of CPA in wheat, peanuts and rice varied from 70% to 111%, 77% to 116% and 69% to 92%, respectively. The method was successfully applied to the analysis of feed and rice samples artificially infected with the fungal strain Penicillium commune, where the toxin was found at different levels.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library