Hyper production of alkali stable xylanase in lesser duration by Bacillus pumilus SV-85S using wheat bran under solid state fermentation
2011
Nagar, Sushil | Mittal, Anuradha | Kumar, Davender | Kumar, Lalit | Kuhad, Ramesh Chander | Gupta, Vijay Kumar
High level production of an extracellular cellulase-poor alkali stable xylanase has been conceded from newly isolated Bacillus pumilus SV-85S under solid state fermentation using wheat bran as a substrate. Optimization of the fermentation conditions enhanced the enzyme production to 73,000±1000IU/g dry substrate, which was 13.8-fold higher than unoptimized conditions (5300IU/g). The enzyme titre was highest after 48h of incubation at 30°C with 1:3 ratios of substrate to moistening agent using wheat bran as a carbon source. The enzyme could be produced in significant levels by using either tap water or distilled water alone as a moistening agent. An elevated production of xylanase by B. pumilus SV-85S in the presence of wheat bran, a cheap and easily available agro-residue, in shorter duration would apparently reduce the enzyme cost substantially. The enzyme was completely stable over a broad pH (5–11) range and retained 52% of its activity at a temperature of 70°C for 30min. The desired characteristics of this enzyme together with economic production would be important for its application in paper and pulp industry.
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