Structural analysis of the lipopolysaccharide from the nontypable Haemophilus influenzae strain SB 33
2001
Cox, Andrew D. | Masoud, Hussein | Thibault, Pierre | Brisson, JeanâRobert | van der Zwan, Mark | Perry, Malcolm B. | Richards, James C.
The structure of the core region of the lipopolysaccharide (LPS) from the nontypable Haemophilus influenzae strain SBâ33 was elucidated. The LPS was subjected to a variety of degradative procedures. The structures of the derived oligosaccharide products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. These analyses revealed a series of related phosphocholine (PCho) containing structures differing in the number of hexose residues. The results pointed to each species containing a conserved phosphoethanolamine (PEtn) substituted heptoseâcontaining trisaccharide innerâcore moiety. The major LPS glycoforms were identified as 2âHex, 3âHex and 4âHex species according to the number of hexose residues present.
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