Optimized McCleary method for measurement of total beta-amylase in barley and its applicability
1996
Santos, M.M.M. | Riis, P.
The McCleary method for determination of beta-amylase in malt has been modified in order to allow determination of total beta-amylase in barley as well as malt. A ruggedness test, performed on the modified method, demonstrated that the method is quite robust and highly reproducible. When the variable, alpha-amylase, beta-amylase and diastatic power were measured in 90 malt samples, only beta-amylase was significantly correlated to diastatic power (r2 = 0.85 and p < 0.0001). The same high correlation was found between total beta-amylase in 20 barley samples and diastatic power in the corresponding malts. The validity of this relationship was tested by predicting diastatic power in malt from total beta-amylase in barley. Predicted values correlated highly to measured values (r2 = 0.95). In breeding material a positive relationship was found between total beta-amylase in barley and protein content. This relationship must be considered when evaluating new barley lines.
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