Effect of ionizing radiation on cholesterol in aqueous dispersion
Lakritz, L. | Maerker, G.
Aqueous sodium stearate dispersions of cholesterol were irradiated at 0-2 degrees C with absorbed doses ranging from 2.5 to 50 kGy. The resulting mixture of cholesterol derivatives was isolated and examined for 7-ketocholesterol and cholesterol 5 alpha, 6 alpha-epoxide and 5 beta, 6 beta-epoxide content. Concentrations of all three compounds increased with dose, while the ratio of 7-ketocholesterol to total epoxides decreased with increasing dose. The ratio of 7-ketocholestrol to the epoxides was approximately 1 or below at all dose levels while the same ratio in autoxidations of cholesterol in dispersions was normally 6 or greater. The change in the keto/epoxide ratio may be a means for determining whether meat or other foods containing cholesterol have been subjected to ionizing radiation.
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