Malachite green method compared to ascorbic acid for estimating small amounts of phosphorus in water, 0.01M calcium chloride, and olsen soil extracts
Subba Rao, A. | Reddy, K.S. | Takkar, P.N.
Malachite green method has been evaluated for estimating minute quantities of phosphorus (P) in water, 0.01 M calcium chloride (CaCl2), and Olsen [sodium bicarbonate (NaHCO3)] extracts of ten soils of varying properties. The precision and accuracy of the malachite green method was better than that of the ascorbic acid method to estimate P in these three extracts. The recovery of added P ranged from 95.3-99.3% in water, 96-100% in 0.01 M CaCl2, and 96.7-100% in Olsen extracts. The ascorbic acid method underestimated the amounts of 0.01 M CaCl2-P and its precision was also lower than the malachite green method. It is possible to predict the P concentration in the three extracts equal to malachite green method from the ascorbic acid P values by using regression equations.
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