Isolation and characterization of high protein and phycocyanin producing mutants of Arthrospira platensis
2018
Shirnalli, Geeta G. | Kaushik, Manish S. | Kumar, Ajay | Abraham, Gerard | Singh, Pawan K.
Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N‐methyl‐Ń‐Nitro‐nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G‐1, G‐2, and SF) were selected and characterized. The G‐1 and G‐2 were helical, more bluish in pigmentation than the wild type strain where G‐1 also showed enlarged cell size. The SF mutant was an altered straight‐filament having maximum biomass. Among three mutants, higher protein and phycocyanin contents were observed in G‐1 and G‐2 mutants whereas chlorophyll was less in these mutants as compared to wild type strain indicating change in the pigment ratio. Carotenoid content was higher in SF mutant as compared to wild type and other mutants. Variation in total sugar content was not observed in comparison to wild type strain. The analysis of amino acid spectrum of all the mutants and wild type showed significant increase in proline content. Overall, it is revealed from the results that G‐1 and G‐2 mutants showed higher biomass, phycocyanin, and protein contents in comparison to wild type which indicated their great potential to be used in food and pharmaceutical industries.
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