Sequential immunohistochemical detection of infectious bursal disease virus antigen in experimental chicken
Hemalatha, S. | Manohar, B Murali | Balachandran, C.
The distribution of infectious bursal disease (IBD) virus antigen in experimentally infected lymphoid and non lymphoid formalin fixed paraffin embedded tissues were studied sequentially from 0 hours post infection (HPI) to 28 days post infection (DPI)using immunoperoxidase (IPT) and immunofluoresence (IFT) staining techniques. A scoring pattern was adopted to study the distribution of viral antigen histologically in the tissues.The viral antigen was noticed in the cortical lymphocytes and plical epithelium of bursa of Fabricius, thymus, spleen, caecal tonsils, Harderian gland within 12 HPI. The macrophages present in interfollicular stromal tissue of bursa, proventriculus and liver showed presence of viral antigen by 24 HPI. The intensity of reaction decreased by 10 DPI to 14 DPI in bursa of Fabricius, thymus and spleen. The antigen started disappearing from Harderian gland, proventriculus, spleen and thymus in that order and persisted for longer time in bursa of Fabricius and caecal tonsils.
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