Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves
Pi, Kai | Luo, Wen | Mo, Zejun | Duan, Lili | Ke, Yuzhou | Wang, Pingsong | Zeng, Shuaibo | Huang, Yin | Liu, Renxiang
BACKGROUND: Potassium(K⁺) plays a vital role in improving the quality of tobacco leaves. However, how to improve the potassium content of tobacco leaves has always been a difficult problem in tobacco planting. K⁺ content in tobacco hybrid is characterized by heterosis, which can improve the quality of tobacco leaves, but its underlying molecular genetic mechanisms remain unclear. RESULTS: Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K⁺ transport (KAT1/2, GORK, AKT2, and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K⁺ homeostasis and heterosis in tobacco leaves. CONCLUSIONS: K⁺ homeostasis and accumulation in tobacco hybrids were collectively improved. The overdominant expression of K⁺ transport and homeostasis-related genes conducted a crucial role in the heterosis of K⁺ content in tobacco leaves.
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