Analysis of fumonisin B1 in rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection
Holcomb, M. | Thompson, H.C. Jr | Hankins, L.J.
A new, sensitive, and stable method has been developed for the analysis of fumonisin B1 in rodent feed. Fumonisin B1, which is the major fumonisin metabolite produced by the fungus Fusarium moniliforme, has been implicated in human and animal diseases. Fumonisin B1 was extracted from spiked rodent feed with acetonitrile/water (50/50) and then cleaned up with tandem C18 Sep-Pak Vac and strong anion-exchange (SAX) columns. Fumonisin B1 was eluted off the SAX column with 1% acetic acid in methanol and quantitated via gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection. The minimum detectable amount in the rodent feed was 0.2 ppm. Recovery values in spiked rodent feed averaged 83% over the range 2-20 ppm.
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