Development of clubroot resistant cabbage line through introgressing six CR loci from Chinese cabbage via interspecific hybridization and embryo rescue
2022
Song, Samnang | Hong, Jeong-Eui | Hossain, Mohammad Rashed | Jung, Hee-Jeong | Nou, Ill-Sup
Multiple R-gene is required to achieve strong resistance against clubroot, a disease caused by Plasmodiophora brassicae in Cabbage (Brassica oleracea, 2n=18, CC). Most of the R-genes were identified in Chinese cabbage (B. rapa, 2n=20, AA), which could be transferred to cabbage via inter interspecific hybridization. We present a breeding scheme to transfer six major CR-loci namely, Crr1, Crr2, Crr3, CRa, CRb and CRc from Chinese cabbage line, LCR36 to cabbage line, Plimio. Approximately, 48% of the interspecific hybrid embryos were rescued, of which chromosomes of 48.15% colchiploid plants were successfully doubled. The hybridity of the colchiploid interspecific hybrids were confirmed by diagnostic sub-genome specific Conserved Ortholog Set (COS) markers, ploidy level, pollen fertility and meiotic chromosome number analyses. Backcross generations are characterized by very low seed set (0.57-1.33% success rate) in backcrossed generations (using Plimio as the recurrent parent). Marker assisted selection using the six CR-loci specific markers identified the resistant plants in F₁ and backcross generations. MAS and bioassay test of BC₂F₂ plants revealed that plants having all six R-loci are resistant to clubroot. Development of subsequent backcross generations are in progress. More number of back-crosses and/or embryo rescue will be performed to ensure sufficient seed set. The success so far is the first report of successful introgression of multiple R-genes via interspecific hybridization in the initial backcrossed generations, indicating that an elite cabbage line having strong resistance to clubroot can be produced upon the completion of the breeding scheme.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library