Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China
2012
Huo, Shuhao | Wang, Zhongming | Zhu, Shunni | Zhou, Weizheng | Dong, Renjie | Yuan, Zhenhong
Cultivation of Chlorella zofingiensis and nutrients removal in dairy wastewater were investigated in bench-scale outdoor ponds in winter, South China. The impacts of the two types of pH regulations, 5∼6% CO₂ and acetic acid (HAc) on this process were studied. After 6days cultivation, the removal rates of total nitrogen (TN) and orthophosphate (PO₄ ³⁻) using CO₂ regulation were better than those using HAc. The removal rates of PO₄ ³⁻ and TN were 97.5% and 51.7%, respectively using CO₂ regulation; 79.6% (TN) and 42.0% (PO₄ ³⁻) were obtained using HAc regulation. Higher biomass, protein, sugar content, and stable pH control were found using CO₂ regulation. However, significantly higher lipid content (31.8%) was observed using HAc regulation. The dominant differences of fatty acids were the content of C18:1 and C18:3. The growth characteristics and environmental conditions especially during the typical logarithmic phase were also analyzed.
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