Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE): Site characterization and on-site bioremediation
Fayolle-Guichard, Françoise | Durand, Jonathan | Cheucle, Mathilde | Rosell, Mónica | Michelland, Rory Julien | Tracol, Jean-Philippe | Le Roux, Françoise | Grundman, Geneviève | Atteia, Olivier | Richnow, Hans H. | Dumestre, Alain | Benoit, Yves
Ethyl tert-butyl ether (ETBE) was detected at high concentration (300mgL⁻¹) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE: 0.91 and 0.83mgL⁻¹h⁻¹ and BTEX: 0.64 and 0.82mgL⁻¹h⁻¹, respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74mgL⁻¹). An on-site pilot plant (2m³) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5×10⁶ copies of ethB gene per L⁻¹.
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